题目内容（请给出正确答案）

[主观题]

Why is a helper phage needed when cloning experiments which a hybrid vector such as pEMBL 8 is done？

A、Efficient insertion

B、Attachment to host

C、To provide replicative enzymes

D、Stable transformation

提问人：网友summertree 发布时间：2022-01-07

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更多“Why is a helper phage needed w…”相关的问题

第1题

What is the most important sequence basis of cosmid construction?

A、Origin of replication is vital

B、Only cos sites are required for packaging

C、Promoter sequences for expression

D、Different restriction sites

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第2题

What does an E.coli genomic library contain?

A、Recombinant clones of genes from E.coli genome

B、Just desired gene from E. coli

C、Expressed genes

D、Protein products

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第3题

Which vectors are mostly used for creating genomic libraries?

A、Lambda phage vectors

B、M13 phage vectors

C、Plasmid vectors

D、Cosmid vectors

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第4题

Which is(are) not the possible reason(s) to use natural selection to isolate the modified lambda that lacks certain restriction sites?

A、Host bacterials that contain restriction enzyme for the Phage strains that contain the retriction sites are almost known.

B、Easier than in vitro mutagenesis

C、There are no restriction sites in lambda

D、Natural selection is less time consuming

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第5题

You know that a strand of template DNA has the following sequence: 5'-ATGGTGCCGGCCTGAAGTCA-3' Which of the following DNA fragments will complement the template DNA, according to Watson-Crick base-pairing rules?

A、3'-TACCACGGCCGGACTTCAGT-5'

B、3'-TAGGACCCGGGGACTTCAGT-5'

C、3'-ATGGTGCCGGCCTGAAGTCA-5'

D、3'-GAGGCACGGCCGGTCAACAGA-5'

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第6题

Which of the following descriptions are NOT true in regard to a cosmid vector?

A、No plaques are produced during the cloning

B、Lac-selection system is used

C、It only carry small DNA fragment

D、Origin of replication is taken from lambda

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第7题

In the laboratory, DNA molecules can be cut at specific sequences using:

A、restriction nucleases

B、UV light

C、DNA ligase

D、laser tweezers

E、DNA polymerase

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第8题

The two strands in the DNA double helix are anti-parallel. What does this mean?

A、One strand runs in the 5' to 3' direction and the other runs 3 to 5 direction

B、Both strands run in the 3' to 5' direction

C、Both strands run in the 5' to 3' direction

D、Anti-parallel is used to refer to the left-handed curve of the DNA double helix

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第9题

Which of the following terms is used to describe the type of RNA that functions as an adaptor, linking amino acids with anticodons?

A、Transfer RNA （tRNA）

B、Messenger RNA （mRNA）

C、MicroRNA（ miRNA）

D、Ribosomal RNA （rRNA）

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第10题

During replication, which enzyme is responsible for making a daughter strand of DNA using the parent strand as template?

A、DNA polymerase

B、Reverse transcriptase

C、RNAse

D、RNA polymerase

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